Identification of drought-responsive genes in roots of upland rice (Oryza sativa L).

Rice (Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. This diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. The analyses of subtractive cDNA libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic control of water use efficiency in rice. Two subtractive libraries were constructed using cDNA of drought susceptible and tolerant genotypes submitted to stress against cDNA of well-watered plants. In silico analysis revealed 463 reads, which were grouped into 282 clusters. Several genes expressed exclusively in the tolerant or susceptible genotypes were identified. Additionally, proteome analysis of roots from stressed plants was performed and 22 proteins putatively associated to drought tolerance were identified by mass spectrometry.

Bibliographic Details

Main Authors:	RABELLO, A. R., GUIMARÃES, C. M., RANGEL, P. H. N., SILVA, F. R. da, SEIXAS, D., SOUZA, E. de, BRASILEIRO, A. C. M., SPEHAR, C. R., FERREIRA, M. E., MEHTA, A.
Other Authors:	ALINE R. RABELLO, UNB; CLEBER MORAIS GUIMARAES, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; F. R. DA SILVA; DANIELA SEIXAS, UFPR; EMANUEL DE SOUZA, UFPR; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CARLOS R. SPEHAR, UNB; MARCIO ELIAS FERREIRA, CENARGEN; ANGELA MEHTA, Cenargen.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2008-11-24
Subjects:	Arroz, Germoplasma, Oryza Sativa, Resistência a Seca, Sistema Radicular, rice,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/217500 https://doi.org/10.1186/1471-2164-9-485
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