Bioassay for detection of transgenic soybean seeds tolerant to glyphosate.

Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 mM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots.

Bibliographic Details

Main Authors:	TORRES, A. C., NASCIMENTO, W. M., PAIVA, S. A. V., ARAGÃO, F. A. S. de
Other Authors:	Antonio Carlos Torres, CNPH; Warley Marco Nascimento, CNPH; Sonia Alessandra Vasconcelos Paiva, CNPH; Fernando Antonio Souza de Aragão, CNPH.
Format:	Artigo de periódico biblioteca
Language:	eng
Published:	2024-10-23
Subjects:	resistência a herbicida, plantas transgênicas, Biossíntese, Glycine Max, biosynthesis, herbicide resistance, transgenic plants,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1168403
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