Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.

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Bibliographic Details
Main Authors: KOLTUN, A., SILVA, P. A. da, TORRES, I. Y., BONIFÁCIO-ANACLETO, F., YASSITEPE, J. E. de C. T.
Other Authors: ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
Format: Artigo de periódico biblioteca
Language:por
Published: 2024-04-05
Subjects:Multiplex PCR, Pontuação alélica manual, Interferência de fluoróforo, Milho tropical, Marcadores microssatélites, Reação em cadeia da polimerase, Manual allele scoring, Pull-up, Fluorophore interference, Tropical maize, Polymerase chain reaction,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303
http://dx.doi.org/10.1590/1984-70332024v24n1a01
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spelling dig-alice-doc-11633032024-04-05T13:35:20Z Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses. KOLTUN, A. SILVA, P. A. da TORRES, I. Y. BONIFÁCIO-ANACLETO, F. YASSITEPE, J. E. de C. T. ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS. Multiplex PCR Pontuação alélica manual Interferência de fluoróforo Milho tropical Marcadores microssatélites Reação em cadeia da polimerase Manual allele scoring Pull-up Fluorophore interference Tropical maize Polymerase chain reaction This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field. 2024-04-05T13:35:20Z 2024-04-05T13:35:20Z 2024-04-05 2024 Artigo de periódico Crop Breeding and Applied Biotechnology, v. 24, n. 1, e46392411, 2024. 1984-7033 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303 http://dx.doi.org/10.1590/1984-70332024v24n1a01 por openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language por
topic Multiplex PCR
Pontuação alélica manual
Interferência de fluoróforo
Milho tropical
Marcadores microssatélites
Reação em cadeia da polimerase
Manual allele scoring
Pull-up
Fluorophore interference
Tropical maize
Polymerase chain reaction
Multiplex PCR
Pontuação alélica manual
Interferência de fluoróforo
Milho tropical
Marcadores microssatélites
Reação em cadeia da polimerase
Manual allele scoring
Pull-up
Fluorophore interference
Tropical maize
Polymerase chain reaction
spellingShingle Multiplex PCR
Pontuação alélica manual
Interferência de fluoróforo
Milho tropical
Marcadores microssatélites
Reação em cadeia da polimerase
Manual allele scoring
Pull-up
Fluorophore interference
Tropical maize
Polymerase chain reaction
Multiplex PCR
Pontuação alélica manual
Interferência de fluoróforo
Milho tropical
Marcadores microssatélites
Reação em cadeia da polimerase
Manual allele scoring
Pull-up
Fluorophore interference
Tropical maize
Polymerase chain reaction
KOLTUN, A.
SILVA, P. A. da
TORRES, I. Y.
BONIFÁCIO-ANACLETO, F.
YASSITEPE, J. E. de C. T.
Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
description This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.
author2 ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
author_facet ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
KOLTUN, A.
SILVA, P. A. da
TORRES, I. Y.
BONIFÁCIO-ANACLETO, F.
YASSITEPE, J. E. de C. T.
format Artigo de periódico
topic_facet Multiplex PCR
Pontuação alélica manual
Interferência de fluoróforo
Milho tropical
Marcadores microssatélites
Reação em cadeia da polimerase
Manual allele scoring
Pull-up
Fluorophore interference
Tropical maize
Polymerase chain reaction
author KOLTUN, A.
SILVA, P. A. da
TORRES, I. Y.
BONIFÁCIO-ANACLETO, F.
YASSITEPE, J. E. de C. T.
author_sort KOLTUN, A.
title Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
title_short Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
title_full Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
title_fullStr Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
title_full_unstemmed Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
title_sort microsatellite markers in maize: challenges and guidelines for implementing multiplex ssr analyses.
publishDate 2024-04-05
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303
http://dx.doi.org/10.1590/1984-70332024v24n1a01
work_keys_str_mv AT koltuna microsatellitemarkersinmaizechallengesandguidelinesforimplementingmultiplexssranalyses
AT silvapada microsatellitemarkersinmaizechallengesandguidelinesforimplementingmultiplexssranalyses
AT torresiy microsatellitemarkersinmaizechallengesandguidelinesforimplementingmultiplexssranalyses
AT bonifacioanacletof microsatellitemarkersinmaizechallengesandguidelinesforimplementingmultiplexssranalyses
AT yassitepejedect microsatellitemarkersinmaizechallengesandguidelinesforimplementingmultiplexssranalyses
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