Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.

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Bibliographic Details
Main Authors: KOLTUN, A., SILVA, P. A. da, TORRES, I. Y., BONIFÁCIO-ANACLETO, F., YASSITEPE, J. E. de C. T.
Other Authors: ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
Format: Artigo de periódico biblioteca
Language:por
Published: 2024-04-05
Subjects:Multiplex PCR, Pontuação alélica manual, Interferência de fluoróforo, Milho tropical, Marcadores microssatélites, Reação em cadeia da polimerase, Manual allele scoring, Pull-up, Fluorophore interference, Tropical maize, Polymerase chain reaction,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303
http://dx.doi.org/10.1590/1984-70332024v24n1a01
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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303
http://dx.doi.org/10.1590/1984-70332024v24n1a01

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