Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.
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Main Authors: | , , , , |
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Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | por |
Published: |
2024-04-05
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Subjects: | Multiplex PCR, Pontuação alélica manual, Interferência de fluoróforo, Milho tropical, Marcadores microssatélites, Reação em cadeia da polimerase, Manual allele scoring, Pull-up, Fluorophore interference, Tropical maize, Polymerase chain reaction, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1163303 http://dx.doi.org/10.1590/1984-70332024v24n1a01 |
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Summary: | This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field. |
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