Meloidogyne incognita control in cotton mediated host-induced gene silencing.

In the present work we used a Host-Induced Gene Silencing (HIGS) strategy employing RNA interference (RNAi) mechanisms in order to silence RKN essential genes involved in development (cathepsin L cysteine proteinase - MiCPL), metabolism (isocitrate lyase - MiICL), mRNA processing (splicing factor - MiSF) and parasitism (nematode effector Mi16D-10). We staked all four RKN genes into a single construct (dsMINC2), driven by the pUceS8.3 constitutive soybean promoter, in order to control the nematode reproduction in transgenic cotton plants developed from an RKN-susceptible cultivar (BRS372). Transgenic dsMinc2-T2 plants infected with Meloidogyne incognita showed a significant reduction in the number of eggs/g.root -1 (73 - 93%) as well as in the reproduction factor (80-93%), compared with the susceptible non-transgenic cultivar.

Bibliographic Details

Main Authors:	SANTOS, M. M. dos, SÁ, M. E. L. de, LEMES, S. V. da R., CAVALCANTE, R. dos S., MORGANTE, C. V., SA, M. F. G. de
Other Authors:	MATEUS MEIRA DOS SANTOS, UnB; MARIA EUGÊNIA LISEI DE SÁ, EPAMIG; SARA VITORINO DA ROCHA LEMES, UnB; RAÍRE DOS SANTOS CAVALCANTE, Bolsista; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen.
Format:	Resumo em anais e proceedings biblioteca
Language:	Portugues pt_BR
Published:	2024-02-27
Subjects:	Nematóide das galhas, Algodão, Gossypium Hirsutum, Doença, Planta Transgênica, Produção Agrícola, Small interfering RNA, Root-knot nematodes, Transgenic plants, Cotton,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162317
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