Large-scale, high-efficiency production of coffee somatic embryos.

The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.

Bibliographic Details

Main Authors:	MACIEL, A. L. de R., RODRIGUES, F. A., PASQUAL, M., CARVALHO, C. H. S. de
Other Authors:	ANNA LYGIA DE REZENDE MACIEL, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO SUL DE MINAS GERAIS; FILIPE ALMENDAGNA RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; MOACIR PASQUAL, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS HENRIQUE S DE CARVALHO, CNPCa.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2024-01-04
Subjects:	Vegetative propagation, Biotechnology, Tissue culture, Coffea arabica var. arabica,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160431 http://dx.doi.org/10.1590/198470332016v16n2a16
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