Production of cellulolytic and hemicellulolytic enzymes by the pectinolytic Aspergillus niger 3T5B8-C88-P83 mutant strain under different cultivation conditions.

Embrapa Agroenergia has been conducting genetic improvement of filamentous fungi to increase the production of cellulases and hemicellulases to deconstruct lignocellulosic biomass. In a conventional breeding program for Aspergillus niger 3T5B8 performed in 2015, after two rounds of mutagenesis (ultraviolet followed by ethyl methanesulfonate), the pectinolytic mutant strain 3T5B8-C88-P83 was selected, which presents genetic changes in the catabolite repression by glucose pathway and a significant increase in polygalacturonase production compared to the original 3T5B8 strain. This work aimed to evaluate the production of cellulolytic and hemicellulolytic enzymes of the 3T5B8-C88-83 mutant under different cultivation conditions, seeking its future application in the enzymatic processing of cotton fabrics. Endoglucanase production by the 3T5B8-C88-P83 mutant strain was evaluated in 250 mL Erlenmeyer flasks containing different liquid and solid culture media (four repetitions).

Bibliographic Details

Main Authors:	CARVALHO, B. A. R., ALVES, L. M., MENDES, T. D., SALUM, T. F. C., FAVARO, L. C. de L.
Other Authors:	BRUNO ANTONIO RIBEIRO CARVALHO; LETICIA MORESCHI ALVES; THAIS DEMARCHI MENDES; THAIS FABIANA CHAN SALUM, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE.
Format:	Resumo em anais e proceedings biblioteca
Language:	Ingles English
Published:	2023-12-28
Subjects:	Mutante, Fermentação, Solido, Enzima Celulolítica, Aspergillus Niger, Polygalacturonase, Mutants, Solid state fermentation,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160301
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