Simulated gastrointestinal digestion/Caco-2 cell model to predict bioaccessibility and intestinal permeability of p-coumaric acid and p-coumaroyl derivatives in peanut.

Data concerning physiological recovery of whole peanut major phenolics throughout the gastrointestinal tract are scarce. In our study, the bioaccessibility and intestinal permeability of peanuts major phenolics were predicted by simulated digestion followed by Caco-2 cells monolayer model. Phenolics identification and quantification were performed by HPLC-ESI-QTOF-MS and HPLC-PDA, respectively. As results, p-coumaroyl conjugates with tartaric, sinapic and ferulic acids, and p-coumaric acid were the major phenolics found in the non-digested extract and in the digested and transported fractions. The in vitro bioaccessibility and Caco-2 cell transport of pcoumaric acid was 370% and 127%, respectively, while it was much lower for p-coumaroyl derivatives (7-100% and 14-31%, respectively). Nonetheless, the peroxyl scavenging activity remained unaltered, likely, at least artly, due to synergies between some phenolics, which concentration proportions changed throughout the experiment. Hence, there is indication that whole peanut is a source of bioavailable antioxidant phenolics.

Bibliographic Details

Main Authors:	MASSARIOLI, A. P., SARTORI, A. G. de O., JULIANO, F. F., AMARAL, J. E. P. G. do, SANTOS, R. C. dos, LIMA, L. M. de, ALENCAR, S. M. de
Other Authors:	ADNA PRADO MASSARIOLI, ESALQ; ALAN GIOVANINI DE OLIVEIRA SARTORI, ESALQ; FERNANDA FRANCETTO JULIANO, ESALQ; JOSÉ EDUARDO PEDROSO GOMES DO AMARAL, ESALQ; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA; SEVERINO MATIAS DE ALENCAR, ESALQ.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2023-03-28
Subjects:	Amendoim, Proteína Vegetal, Nutrição Humana, Óleo Vegetal, Phenolic compounds, Peanuts, Arid zones,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152824
Tags:	Add Tag No Tags, Be the first to tag this record!