Assessment of copy number variants in three Brazilian locally adapted cattle breeds using whole-genome re-sequencing data.

Further characterization of genetic structural variations should strongly focus on small and endangered local breeds given their role in unraveling genes and structural variants underlying selective pressures and phenotype variation. A comprehensive genome-wide assessment of copy number variations (CNVs) based on whole-genome re-sequencing data was performed on three Brazilian locally adapted cattle breeds (Caracu Caldeano, Crioulo Lageano, and Pantaneiro) using the ARS-UCD1.2 genome assembly. Data from 36 individuals with an average coverage depth of 14.07× per individual was used. A total of 24?945 CNVs were identified distributed among the breeds (Caracu Caldeano = 7285, Crioulo Lageano = 7297, and Pantaneiro = 10 363). Deletion events were 1.75?2.07-fold higher than duplications, and the total length of CNVs is composed mostly of a high number of segments between 10 and 30?kb. CNV regions (CNVRs) are not uniformly scattered throughout the genomes (n = 463), and 105 CNVRs were found overlapping among the studied breeds. Functional annotation of the CNVRs revealed variants with high consequence on protein sequence harboring relevant genes, in which we highlighted the BOLA-DQB, BOLA-DQA5, CD1A, ?-defensins, PRG3, and ULBP21 genes. Enrichment analysis based on the gene list retrieved from the CNVRs disclosed over-represented terms (p?<?0.01) strongly associated with immunity and cattle resilience to harsh environments. Additionally, QTL associated with body conformation and dairy-related traits were also unveiled within the CNVRs. These results provide better understanding of the selective forces shaping the genome of such cattle breeds and identify traces of natural selection pressures by which these populations have been exposed to challenging environmental conditions.

Bibliographic Details

Main Authors:	PERIPOLLI, E., STAFUZZA, N. B., MACHADO, M. A., PANETTO, J. C. do C., EGITO, A. A. do, BALDI, F., SILVA, M. V. G. B.
Other Authors:	ELISA PERIPOLLI, Universidade Estadual Paulista; NEDENIA BONVINO STAFUZZA, Centro de Pesquisa em Bovinos de Corte; MARCO ANTONIO MACHADO, CNPGL; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; ANDREA ALVES DO EGITO, CNPGC; FERNANDO BALDI, Universidade Estadual Paulista; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2023-02-10
Subjects:	Variação fenotípica, Bovino, Raça, Gado Crioulo, Genoma,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151656 https://doi.org/10.1111/age.13298
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