Analysis of fungal co-culture metabolites using both classic and imprinting Mass Spectrometry Imaging.

Analysis of fungal co-culture metabolites using both classic and imprinting Mass Spectrometry Imaging.

Fungi are able to produce a wide range of bioactive metabolites, which enhanced when challenged in co-culture[1]. For a better evaluation of these metabolites, the Mass Spectrometry Imaging (MSI) can be used to provide complementary information through the metabolite spatial localization [2]. However, some adaptations are required on available methodologies in MSI for applications in microrganisms[2], particularly on sample preparation. The imprinting method has been shown to be a robust method when applied to preparation of samples analyzed by DESI-MSI[3], but it has never been tested for MALDI-MSI for microrganisms, in our knowledge.

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Bibliographic Details
Main Authors: TEIXEIRA, T. S., RODRIGUES NETO, J. C., CONCEIÇÃO, A. A., COSTA, P. P. K. G., SIQUEIRA, F. G. de, ABDELNUR, P. V.
Other Authors: TALLYTA SANTOS TEIXEIRA, CNPAE; JORGE CANDIDO RODRIGUES NETO, CNPAE; APARECIDO ALMEIDA CONCEIÇÃO, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE.
Format: Anais e Proceedings de eventos biblioteca
Language:Ingles
English
Published: In: IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3., 2022, Rio de Janeiro. Book of abstracts. [s/l]: Sociedade Brasileira de Espectrometria de Massas, 2022. 2023-01-12
Subjects:MALDI-MSI, Pleorotus pulmonarius, Aspergillus terreus, Metabolomics,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150904
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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150904

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