Dietary supplementation of Probiotic Enterococcus faecium improve resistance in Arapaima gigas against Aeromonas hydrophila.

This study evaluated the mortality rate, histopathology and haematological param-eters of Arapaima gigas against Aeromonas hydrophila after 68 days of dietary probiotic supplementation with autochthonous bacterium Enterococcus faecium. Three experi-ments were carried out: the first assay evaluated the lethality of A. hydrophila, the second and the third assay evaluated the fish supplemented subjected to A. hydrophilalethal dose 30% (106 CFU ml−1) and lethal dose 100% (108CFU ml−1) respectively. The clinical signs, blood changes, histopathological alterations and mortalities were evalu-ated. At the first experiment, the concentrations of 106 and 108CFU.mL−1 with A. hy-drophila caused 33% and 100% of mortality. The A. hydrophila infection provoked clinical signs such as dark skin, ulceration, haemorrhage, pale gills and liver, hepatic alterations, hyperaemia, hepatic cord breakdown, cellular deforming, lipid degener-ation and necrosis. In the second experiment, no mortality occurred onto fish fed with probiotic. Furthermore, in the third experiment, fish submitted to probiotic sup-plementation showed reduction in mortality of 75% compared with the control and fish fed with probiotic diets at 108CFU g−1 not presented any clinical signs. For these reasons, Arapaima gigas juveniles previously submitted to probiotic supplementation with E. faecium (108CFU g−1) shows better physiological and immunological response, improving resistance against A. hydrophila infection.

Bibliographic Details

Main Authors:	COUTO, M. V. S. do, SOUSA, N. C., ABE, H. A., CUNHA, F. S., MENESES, J. O., PAIXÃO, P. E. G., MOURINO, J. L. P., MARTINS, M. L., CORDEIRO, C. A. M., FUJIMOTO, R. Y.
Other Authors:	MÁRCIA VALÉRIA SILVA DO COUTO; NATALINO COSTA SOUSA; HIGO ANDRADE ABE; FERNANDA SANTOS CUNHA; JULIANA OLIVEIRA MENESES; PETERSON EMMANUEL GUIMARÃES PAIXÃO; JOSE LUIZ PEDREIRA MOURINO; MAURÍCIO LATERÇA MARTINS; CARLOS ALBERTO MARTINS CORDEIRO; RODRIGO YUDI FUJIMOTO, CPATC.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	Aquaculture Research, v. 53, p. 3453-3463, 2022. 2022-12-22
Subjects:	Peixe, Pirarucu, Hematologia., Doença Animal, Fish, Fish diseases, Hematology.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150243
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