Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | Ingles English |
| Published: |
2021-09-21
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| Subjects: | Bovine semen, Cattle fertility, selenium, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134629 http://dx.doi.org/10.21577/0103-5053.20170089 |
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