Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.

CARRIERO, M. M.; HENRIQUE SILVA, F.; MEIRA, C. M.; GATO, I. M. Q.; CAETANO, A. R.; LOBO, F. P.; ALVES, A. L.; VARELA, E. S.; MAIA, A. A. M.; MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.

Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.

In the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.

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Main Authors:	CARRIERO, M. M., HENRIQUE SILVA, F., MEIRA, C. M., GATO, I. M. Q., CAETANO, A. R., LOBO, F. P., ALVES, A. L., VARELA, E. S., MAIA, A. A. M.
Other Authors:	MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2020-11-11
Subjects:	Bacterial infection, Fish immunology, South American fish, Peixe, Pacu, Infecção, Gene expression, Piaractus mesopotamicus, Fish, Interleukins,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471 https://doi.org/10.1590/1678-4685-gmb-2020-0006
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id	dig-alice-doc-1126471
record_format	koha
spelling	dig-alice-doc-11264712021-01-23T09:08:44Z Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis. CARRIERO, M. M. HENRIQUE SILVA, F. MEIRA, C. M. GATO, I. M. Q. CAETANO, A. R. LOBO, F. P. ALVES, A. L. VARELA, E. S. MAIA, A. A. M. MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP. Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins In the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups. 2021-01-23T09:08:33Z 2021-01-23T09:08:33Z 2020-11-11 2020 Artigo de periódico Genetics and Molecular Biology, v. 43, n. 4, e20200006, 2020. 1678-4685 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471 https://doi.org/10.1590/1678-4685-gmb-2020-0006 Ingles en openAccess
institution	EMBRAPA
collection	DSpace
country	Brasil
countrycode	BR
component	Bibliográfico
access	En linea
databasecode	dig-alice
tag	biblioteca
region	America del Sur
libraryname	Sistema de bibliotecas de EMBRAPA
language	Ingles English
topic	Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins
spellingShingle	Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins CARRIERO, M. M. HENRIQUE SILVA, F. MEIRA, C. M. GATO, I. M. Q. CAETANO, A. R. LOBO, F. P. ALVES, A. L. VARELA, E. S. MAIA, A. A. M. Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
description	In the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.
author2	MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.
author_facet	MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP. CARRIERO, M. M. HENRIQUE SILVA, F. MEIRA, C. M. GATO, I. M. Q. CAETANO, A. R. LOBO, F. P. ALVES, A. L. VARELA, E. S. MAIA, A. A. M.
format	Artigo de periódico
topic_facet	Bacterial infection Fish immunology South American fish Peixe Pacu Infecção Gene expression Piaractus mesopotamicus Fish Interleukins
author	CARRIERO, M. M. HENRIQUE SILVA, F. MEIRA, C. M. GATO, I. M. Q. CAETANO, A. R. LOBO, F. P. ALVES, A. L. VARELA, E. S. MAIA, A. A. M.
author_sort	CARRIERO, M. M.
title	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
title_short	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
title_full	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
title_fullStr	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
title_full_unstemmed	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
title_sort	molecular characterization and gene expression analysis of the pro-inflammatory cytokines il-1beta and il-8 in the south american fish piaractus mesopotamicus challenged with aeromonas dhakensis.
publishDate	2020-11-11
url	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471 https://doi.org/10.1590/1678-4685-gmb-2020-0006
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Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.

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