Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.
In the present study, the complete characterization of cDNA and genomic sequences of IL-1B and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1B cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1B, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1B and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | Ingles English |
| Published: |
2020-11-11
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| Subjects: | Bacterial infection, Fish immunology, South American fish, Peixe, Pacu, Infecção, Gene expression, Piaractus mesopotamicus, Fish, Interleukins, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126471 https://doi.org/10.1590/1678-4685-gmb-2020-0006 |
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