Osmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).

The study aimed to establish the most suitable osmolarity range of the hypoosmotic solution for testing the membrane integrity in raw semen of rams. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/L) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/L (S10). Nine mature Santa Ines rams (21.5+0.5 months; 72.9+1.6 kg) had semen collected one month apart. After the hypoosmotic swelling test (HOST) the ejaculates were evaluated using a phase-contrast microscope. A total of 200 spermatozoa were counted and sperm cells were classified as spermatozoa with coiled tails (CD, %), strongly coiled tail (CFD, %) or non-coiled tails. According to CD, S1, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). According to CED, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). Results suggest that the 75 up to 200 mOsm/L solution would be bestfor using in HOST when raw semen of rams is analyzed.

Bibliographic Details

Main Authors:	GARCIA, A. R., MOURA, A. B. B., ROMANELLO, N., FONSECA, J. F. da, BRANDÃO, F. Z., PANTOJA, M. H. de A., BARRETO, A. do N., SOUSA, M. A. de P., BOTTA, D., GIRO, A.
Other Authors:	ALEXANDRE ROSSETTO GARCIA, CPPSE; Ana Beatriz Bossois Moura, UFF; Narian Romanello, USP; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF; Messy Hannear de Andrade Pantoja, USP; Andréa do Nascimento Barreto, UFPA; Marco Antonio de Paula Sousa, UFPA; Daniela Botta, UFPA; Alessandro Giro, UFPA.
Format:	Anais e Proceedings de eventos biblioteca
Language:	pt_BR pt_BR
Published:	2019-12-12
Subjects:	Solução hiposmótica, Hypoosmotic solution, Ram, Sperm, Ovino, Espermatozóide,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116740
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