A host specialized form of Ceratocystis fimbriata causes seed and seedling blight on native Carapa guianensis (andiroba) in Amazonian rainforests.

Ceratocystis fimbriata Ellis & Halsted recently was recorded causing seed and seedling blight on Carapa guianensis Aubl. (andiroba), a tree species native to the Amazon Rainforest and prized for its valuable timber and medicinal seed oil. C. fimbriata more commonly causes wilt type diseases in woody hosts, especially on non-native host trees. However, on andiroba the disease occurs on seedlings and seeds, affecting the species regeneration. We studied 73 isolates of C. fimbriata on andiroba from three regions of the Amazon Basin to see if they represented natural or introduced populations. Analysis of ITS rDNA sequences and phylogenetic analysis of mating type genes revealed new haplotypes of C. fimbriata from the Latin American Clade that were closely related to other Brazilian populations of the fungus. In mating experiments, andiroba isolates were inter-fertile with tester strains of C. fimbriata from Brazil and elsewhere, confirming that they belong to a single biological species. Using microsatellite markers, 14 genotypes and populations with intermediate levels of genetic variability were found, suggesting that the fungus is indigenous to the Amazon Basin. Inoculation tests indicated that the andiroba isolates are host-specialized on andiroba, supporting the proposition of the special form C. fimbriata f. sp. carapa.

Bibliographic Details

Main Authors:	VALDETARO, D. C. O. F., HARRINGTON, T. C., OLIVEIRA, L. S. S., GUIMARÃES, L. M. S., MCNEW, D. L., PIMENTA, L. V. A., GONCALVES, R. C., SCHURT, D. A., ALFENAS, A. C.
Other Authors:	Denise C. O. F. Valdetaro, Universidade Federal de Viçosa; Thomas C. Harrington, Iowa State University; Leonardo S. S. Oliveira, Universidade Federal de Viçosa; Lúcio M. S. Guimarães, Universidade Federal de Viçosa; Douglas L. McNew, Iowa State University; Lucas V. A. Pimenta, Universidade Federal de Viçosa; RIVADALVE COELHO GONCALVES, CPAF-AC; DANIEL AUGUSTO SCHURT, CPAF-RR; Acelino C. Alfenas, Universidade Federal de Viçosa.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2019-03-19
Subjects:	Produto florestal não madeireiro (PFNM), Enfermedades fungales de las plantas, Plantas de semilleros, Semillas, Variación genética, Repeticiones de microsatélite, Marcador microssatélite, Stem blight, Tizón del tallo, Andiroba, Carapa Guianensis, Doença Fúngica, Fungo, Ceratocystis Fimbriata, Plântula, Semente, Variação Genética, Marcador Genético, Nontimber forest products, Fungal diseases of plants, Seedlings, Seeds, Genetic variation, Genetic markers, Microsatellite repeats,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107239
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