Anormalidades morfológicas e danos no DNA espermático em touros bubalinos (Bubalus bubalis) submetidos à insulação testicular.

e aim was to understand the performance of testicular thermal stress on the morphology, compaction and integrity of DNA in the different phases of the spermatogenic cycle in buffaloes, using chromomycin A3 and acridine orange respectively. Five adult bulls undergoing testicular insulation for 48 hours and semen collected once every seven days, divided into pre and post testicular insulation. e post-insulation testicular temperature increased 3.2ºC (p<0.05) in relation to the control. ere was an increase (p<0.05) in the deprotamination of the spermatic chromatin in the seventh week a#er insulation compared to the control (11.0 ±3.1 and 3.0 ±2.8), but there was no difference in DNA fragmentation a#er insulation. However, head defects showed an increase (p<0.05) in the seventh week in relation to the control (0.7 ±0.2 and 0.13 ±0.16). erefore, the increase in testicular temperature in buffaloes had a negative influence on spermatogonia and primary spermatocytes on chromatin protamination.

Bibliographic Details

Main Authors:	GONÇALVES, A. A., MARIN, D. F. D., SILVA, T. V. G., LIMA, A. J. M. de, MORAIS, M. A. R., SOUZA, E. B. de, MELO, D. N. de, ROLIM FILHO, S. T., GARCIA, A. R., OHASHI, O. M.
Other Authors:	Arnaldo Algaranhar Gonçalves, UFPA; Diego Fernando Dubeibe Marin, UFPA; Tiago Velasco Guimarães Silva, UFPA; Ana Júlia Mota de Lima, UFPA; Mauro Andrey Rodrigues Morais, UFPA; Eduardo Baia de Souza, UFPA; Dayana Neves de Melo, Universidade Federal Rural da Amazônia; Sebastião Tavares Rolim Filho, Universidade Federal Rural da Amazônia; ALEXANDRE ROSSETTO GARCIA, CPPSE; Otávio Mitio Ohashi, Universidade Federal Rural da Amazônia.
Format:	Separatas biblioteca
Language:	pt_BR por
Published:	2018-12-12
Subjects:	Cromatina, Fragmentação, Protamina, Bubalus Bubalis,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101231
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