Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1.

In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1

Bibliographic Details

Main Authors:	SILVA, J. M. F, RWAHNIH, M. A., BLAWID, R., NAGATA, T., FAJARDO, T. V. M.
Other Authors:	João Marcos Fagundes Silva, University of Bras?lia, Bras?lia, DF, Brazil; Maher Al Rwahnih, Department of Plant Pathology, University of California, Davis, CA, USA; Rosana Blawid, University of Bras? ? lia, Bras? ? lia, DF, Brazil; Tatsuya Nagata, University of Bras?lia, Bras?lia, DF, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2017-06-27
Subjects:	High-throughput sequencing, Grapevine enamovirus-1, GEV-1., Luteoviridae.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071596
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