Occurrence of apple stem grooving capillovirus in Santa Catarina, Brazil, detected by RT-PCR.
Apple stem grooving capillovirus (ASGV) was detected in apple plants in Fraiburgo, Santa Catarina State. The virus was isolated from the cultivars 'Royal Gala' and 'Fuji' grafted onto 'Maruba-kaido", with an MM106 interstock between rootstock and canopy. Two nurseries involving several thousand plants were heavily affected by the virus and had to be destroyed. Declining plants showed tissue necrosis in the 'Maruba-kaido' region around the stem. The virus was readily transmitted to Chenopodium quinoa from flower petals and young leaves of affected plants. In mechanically inoculated Nicotiana occidentalis 37B infection was latent. Virus identification was performed by RT-PCR and ISEM. Key words: stem grooving, apple, identification, PCR, silica capture. Apple stem grooving capillovirus (ASGV) foi detectado em macieiras cv. 'Royal Gala' e 'Fuji' sobre portaenxerto 'Maruba-kaido' com um inter-enxerto de MM 106 entre a copa e o porta-enxerto. Dois viveiros envolvendo milhares de plantas foram afetados severamente e tiveram que ser destruídos. Macieiras em declínio apresentavam necrose da casca no porta-enxerto 'Maruba' ao redor do tronco. O vírus foi facilmente transmitido mecanicamente de pétalas e folhas jovens de macieiras afetadas para C. quinoa e desta para N. occidentalis cv. 37B, na qual o isolado produziu reação latente. A identificação do vírus foi feita por RT-PCR e ISEM.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | English eng |
| Published: |
2016-09-23
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| Subjects: | Stem grooving, Apple, Identification, PCR, Silica capture, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053382 |
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