An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry.

In vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA).

Bibliographic Details

Main Authors:	NOGUEIRA, G. F., PIO, L. A. S., PASQUAL, M., AMARAL, A. L. do, CHERWINSKI-PEREIRA, J. E.
Other Authors:	GABRIELA FERREIRA NOGUEIRA; LEILA APARECIDA SALLES PIO, UFLA; MOACIR PASQUAL, UFLA; ADRIANE LEITE DO AMARAL, CPATC; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN.
Format:	Separatas biblioteca
Language:	English eng
Published:	2015-09-14
Subjects:	Cana de açúcar, Flow cytometry, Sugarcane,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023802
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