Fusarium verticillioides MAT1-2 strain near isogenic to the sequenced FGSC7600

The study goal was to create a Fusarium verticillioides MAT1-2 strain that was near isogenic to the sequenced FGSC 7600 (NRRL 20955). We accomplished this by using the sexual cycle of the fungus to cross FGSC 7600, carrying the MAT1-1 locus, with FGSC7603 (NRRL 20956), carrying the MAT1-2 locus. Progeny with the MAT1-2 locus were sequentially backcrossed with FGSC 7600 six times. We sequenced each generation with a MiSeq platform and then mapped recombination events. The final MAT1-2 strain is approximately 93% identical to FGSC7600 and is available from the Fungal Genetics Stock Center (FGSC 27326) and from the USDA ARS Culture Collection as NRRL 64809.

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Bibliographic Details
Main Author: USDA-ARS (17854919)
Format: Dataset biblioteca
Published: 2024
Subjects:Genetics, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/Fusarium_verticillioides_MAT1-2_strain_near_isogenic_to_the_sequenced_FGSC7600/27123135
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