Leaf endomicrobiomes of early and late planted sorghum seedlings
Leaf fungal and bacterial communities from field-grown sorghum accessions (KS and SD) were characterized in response to chilling and non-chilling temperatures using amplicon sequencing. Bacterial primers 799F and 1115R were used to amplify 16S V5-V6. Fungal primers ITS1f and ITS2 were used to amplify ITS1. Locus-specific PCR was followed by index PCR with Illumina Nextera Kit v2 sets A, B, C, D. After PCR clean-up, amplicons were pooled equimolarly and sequenced on an Illumina MiSeq 2 x 300 at South Dakota State University Genomics Sequencing Center.
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| Format: | Dataset biblioteca |
| Published: |
2023
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/Leaf_endomicrobiomes_of_early_and_late_planted_sorghum_seedlings/25091093 |
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