The First Gene-Edited Calf with Resistance to a Major Bovine Viral Disease
Bovine viral diarrhea virus (BVDV) is one of the most important viruses affecting the health and well-being of bovine species throughout the world. Here we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by clinical signs, and the lack of viral infection in white blood cells. The edited calf has no off-target edits and appears normal and healthy at one year of age without obvious adverse effects from the on-target edit. This precision bred, proof-of-concept animal provides the first evidence that intentional genome alterations may reduce the burden of BVDV-associated diseases in cattle, and is consistent with our stepwise, in vitro and ex vivo experiments with cell lines and matched fetal clones.
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| Format: | Dataset biblioteca |
| Published: |
2022
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/The_First_Gene-Edited_Calf_with_Resistance_to_a_Major_Bovine_Viral_Disease/25090553 |
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