Real-time quantitative PCR analysis of gene expression in human colon cancer cells treated with cottonseed-derived gossypol
Relative mRNA levels of 55 genes were evaluated with the SYBR Green qPCR assays after gossypol treatment for 8 h. Overall design: Human colon cancer cells (COLO 225) were purchased from ATCC. The cells in triplicate were treated with various concentrations of gossypol for 8 h. RNA isolation and cDNA synthesis were performed as described previously. Relative mRNA levels were evaluated with the SYBR Green qPCR assays using Bio-Rad reagents. BCL2 mRNA was selected as the internal reference for qPCR analysis for this cell type. 1% DMSO treatment was the sample control. The 2-ΔCT and 2-ΔΔCT method of relative quantification was used to determine the fold change in expression.
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| Format: | Dataset biblioteca |
| Published: |
2022
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| Subjects: | Genetics, Homo sapiens, eEukaryotes, |
| Online Access: | https://figshare.com/articles/dataset/Real-time_quantitative_PCR_analysis_of_gene_expression_in_human_colon_cancer_cells_treated_with_cottonseed-derived_gossypol/25089962 |
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