Triticum turgidum strain:Kronos Transcriptome or Gene expression
For this project, we have sequenced, assembled and annotated a transcriptome of a tetraploid wheat T. turgidum cv. Kronos. The sequencing libraries were prepared from root and shoot tissues from young 2-3 week old seedlings, developing spikes at the booting stage and grain 20 days post anthesis. An additional library from multiple tissues was normalized with double-stranded DNA nuclease. All sequencing was carried out on the Illumina HiSeq platform using the 100 bp paired-end protocol (562.4 million reads). The assembly was constructed using a multiple k-mer approach with a de novo assembly algorithm implemented in CLC Genomics Workbench 5.5 and additional redundancy reduction with CD-HIT and blast2cap3 programs. Open reading frames and proteins were predicted using BLASTX searches and a findorf algorithm.
| Main Authors: | , |
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| Format: | Dataset biblioteca |
| Published: |
2013
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| Subjects: | Genetics, Triticum turgidum, Kronos, eEukaryotes, transcriptome, |
| Online Access: | https://figshare.com/articles/dataset/Triticum_turgidum_strain_Kronos_Transcriptome_or_Gene_expression/25079330 |
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