ChIP-seq of bovine cells (MDBK) to study butyrate-induced histone modification
Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4 Overall design: Cells were treated with 10 mM butyrate for 24 hr, The cells were scraped from the flask and homogenized with ice-cold Dounce homogenizer to release the nuclei. The collected nuclei were resuspended in digestion buffer and enzymatic shearing was performed. ChIP with anti H3, H4 and acetyl-H3 and acetyl-H4.
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| Main Author: | |
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| Format: | Dataset biblioteca |
| Published: |
2012
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| Subjects: | Genetics, Bos taurus, eEukaryotes, |
| Online Access: | https://figshare.com/articles/dataset/ChIP-seq_of_bovine_cells_MDBK_to_study_butyrate-induced_histone_modification/25079141 |
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