Zea mays Transcriptome or Gene expression

The experiment consisted of a field design containing two maize inbred lines Va35 and Mp313E. Va35 has yellow kernels and is susceptible to kernel infection by Aspergillus flavus (A. flavus). Mp313E is a white dent maize inbred line and was released primarily as a source of resistance to kernel infection by A. flavus. The test ears were inoculated with Aspergillus flavus and collected two days after inoculation. Non-inoculated ears collected 16 days after pollination were also used as a control. The microarray experimental design was a randomized complete block design with three replications. One microarray slide was used for each field plot. Each slide contained the inoculated and non-inoculated sub-treatments. A second slide for one replication of each genotype contained a dye swap for the inoculation treatment and three subsamples (dots) within the slide represented each contig. Keywords: Direct comparison Overall design: Two-condition experiment, inoculated vs. uninoculated maize ears for Va35 and Mp313E. Biological replicates: 3 control, 3 inoculated, 2 dye-swap, independently grown and harvested. One replicate per array.

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Bibliographic Details
Main Author: Maize Genetics, Corn Host Plant Resistance Research Unit (CHPRRU), USDA-ARS (18794827)
Format: Dataset biblioteca
Published: 2007
Subjects:Genetics, Zea mays, eEukaryotes,
Online Access:https://figshare.com/articles/dataset/Zea_mays_Transcriptome_or_Gene_expression/25078724
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