Data from: Control of chrysanthemum flowering through integration with an aging pathway
<p>Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures flowering independent of environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing the expression of a nuclear factor gene, <em>CmNF-YB8</em>, from the short day plant chrysanthemum (<em>Chrysanthemum morifolium</em>), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions. The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, <em>SPL3</em>, <em>SPL5</em>, and <em>SPL9</em>, is upregulated in <em>CmNF-YB8</em>-RNAi plants, while expression of the microRNA, <em>cmo-MIR156</em>, is downregulated. In addition, <em>CmNF-YB8</em> is shown to bind to the promoter of the <em>cmo-MIR156</em> gene. Ectopic expression of <em>cmo-miR156</em>, using a virus-based microRNA expression system, restores the early flowering phenotype caused by <em>CmNF-YB8</em> silencing. These results show that <em>CmNF-YB8</em> influences flowering time through directly regulating the expression of <em>cmo-MIR156</em> in the aging pathway.</p> <p>RNA-seq data associated with this study has been deposited in NCBI SRA database under accession code PRJNA393366. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Supplementary Data 1 - Differentially transcribed genes in CmNF-YB8-RNAi plants compared with wild type chrysanthemum .</p> <p>File Name: Web Page, url: <a href="https://static-content.springer.com/esm/art:10.1038/s41467-017-00812-0/MediaObjects/41467_2017_812_MOESM2_ESM.xlsx">https://static-content.springer.com/esm/art:10.1038/s41467-017-00812-0/MediaObjects/41467_2017_812_MOESM2_ESM.xlsx</a> </p><p>Provides Accession, Annotation, and Fold Change (Excel download)</p></li></ul><p></p>
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Dataset biblioteca |
| Published: |
2019
|
| Subjects: | Crop and pasture production, Genetics, Gene expression (incl. microarray and other genome-wide approaches), Plant biology not elsewhere classified, aging pathway, flower timing, flowering pathway, RNA-Seq, Chrysanthemum, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Control_of_chrysanthemum_flowering_through_integration_with_an_aging_pathway/24853404 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|