Data from: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay

<p>A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (<em>Oncorhynchus mykiss</em>), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for <em>drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a-like-b, tnfrsf5</em>, and <em>tnfrsf9</em>. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and the <em>Flavobacterium psychrophilum</em> load are reported. These data are an extension of information presented and discussed in the article "Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with <em>Flavobacterium psychrophilum</em> in resistant and susceptible lines of rainbow trout (<em>Oncorhynchus mykiss</em>)" (Kutyrev et al., 2016). </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay.</p> <p>File Name: Web Page, url: <a href="https://www.sciencedirect.com/science/article/pii/S2352340917300331">https://www.sciencedirect.com/science/article/pii/S2352340917300331</a> </p><p><em>Data in Brief</em> data article providing expression values for a suite of immune-relevant genes in rainbow trout (<em>Oncorhynchus mykiss</em>), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Data are available for download as Excel spreadsheet.</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://office.microsoft.com/excel/">https://office.microsoft.com/excel/</a> </p></li></ul><p></p>

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Bibliographic Details
Main Authors: Ivan A. Kutyrev (17478765), Beth M. Cleveland (17478768), Timothy D. Leeds (17478771), Gregory D. Wiens (17478774)
Format: Dataset biblioteca
Published: 2018
Subjects:Animal production, Genetics, Gene expression (incl. microarray and other genome-wide approaches), Animal structure and function, proinflammatory cytokine, cytokine receptor gene expression, GeXP multiplex RT-PCR assay, data.gov, ARS,
Online Access:https://figshare.com/articles/dataset/Data_from_Dataset_of_proinflammatory_cytokine_and_cytokine_receptor_gene_expression_in_rainbow_trout_Oncorhynchus_mykiss_measured_using_a_novel_GeXP_multiplex_RT-PCR_assay/24852609
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