Data from: Dataset on characterization of recombinant interleukin-23α, IL-12p40 and IL-23 complex protein, which activates JAK-STAT signaling pathway in chicken cell lines using immunocytochemical staining
<p>The data herein is related to the research article entitled "Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex", where it is demonstrated that the chicken interleukin (IL)-23α, IL-12p40, and IL-23 complex regulates Th1, Th17, and Treg cytokine production through heterodimer receptors as well as a homodimer receptor consisting of IL-12Rβ1 and IL-23R, and activates the JAK/STAT signaling pathways. Here, the effects of the recombinant chicken IL-23α, IL-12p40, and IL-23 complex protein on cell proliferation and nitric oxide (NO) production in chicken macrophage (HD11) and CU91 T cell lines are evaluated. In addition, the expression of IL-6, IL-17A, and interferon-γ mRNA were upregulated <em>in vivo</em> and <em>in vitro</em>. Moreover, treatment with the chicken IL-23α, IL-12p40, and IL-23 complex activated phosphorylation of tyrosine and serine residues in JAK2, STAT1, TYK2, and SOCS1 in chicken cell lines. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Dataset on characterization of recombinant interleukin-23α, IL-12p40 and IL-23 complex protein, which activates JAK-STAT signaling pathway in chicken cell lines using immunocytochemical staining.</p> <p>File Name: Web Page, url: <a href="https://www.sciencedirect.com/science/article/pii/S2352340917307035">https://www.sciencedirect.com/science/article/pii/S2352340917307035</a> </p><p><em>Data in Brief</em> article providing the nucleotide and amino acid sequences of the chicken IL-23α coding region. The data provide information about the effect of chicken IL-23α, IL-12p40, and IL-23 complex protein on cell proliferation and production of reactive oxygen species in the form of NO in both cell lines. </p></li></ul><p></p>
| Main Authors: | , , , , , , |
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| Format: | Dataset biblioteca |
| Published: |
2018
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| Subjects: | Animal production, Genetics, Gene expression (incl. microarray and other genome-wide approaches), Animal structure and function, cytokine production, JAK-STAT signaling pathway, data.gov, ARS, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Dataset_on_characterization_of_recombinant_interleukin-23_IL-12p40_and_IL-23_complex_protein_which_activates_JAK-STAT_signaling_pathway_in_chicken_cell_lines_using_immunocytochemical_staining/24852597 |
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