Data from: Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca
<p>All source data from the referenced paper (Figures 1b and Table 1). 22 Excel files of data from each experimental block of the reactive oxygen species assay, 1 Excel file of the combined data from the bacterial growth enhancement assay, 1 Excel file of the RT-qPCR data.</p> <p>Parasitic plants are the primary biotic constraint in many crop production systems. The most agriculturally devastating parasitic plants, including witchweeds (<em>Striga spp.</em>) and broomrapes (<em>Phelipanche and Orobanche spp.</em>), are in the <em>Orobanchaceae</em> family. <em>Phelipanche aegyptiaca</em> is an obligate holoparasite that lacks the capacity for photosynthesis and therefore relies on host parasitization for acquisition of all nutrients. <em>P. aegyptiaca</em> is a broad host range pathogen with the ability to parasitize diverse dicot hosts through attachment and development of a feeding structure known as a haustorium. The mechanisms <em>P. aegyptiaca</em> and other parasitic plants employ to avoid host plant immunity and form successful haustorial attachments are unknown. Here, we demonstrate that <em>P. aegyptiaca</em> actively suppresses salicylic acid-mediated immunity of the host plant <em>Arabidopsis thaliana</em>. We hypothesized that parasitic plants may deploy immunity-suppressing effector proteins through the haustorial interface to subvert host plant immune responses. We devised a pipeline to select and clone 27 candidate secreted effector proteins in <em>P. aegyptiaca</em> and tested these proteins for the potential to suppress known plant immunity pathways. Five candidate effectors suppressed flg22-elicited production of reactive oxygen species when transiently expressed in <em>Nicotiana benthamiana</em>. We propose that two of these candidate effectors function through interfering with pattern triggered immunity using molecular mimicry. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Data from Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca.</p> <p>File Name: AllSourceDataPaegEffectorPaper.zip</p><p>Resource Description: All source data from the referenced paper.</p></li></ul><p></p>
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| Format: | Dataset biblioteca |
| Published: |
2019
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| Subjects: | Host-parasite interactions, Genetics, Environmental sciences, NP303, data.gov, ARS, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Identification_and_functional_characterization_of_immunity-suppressing_candidate_effector_proteins_in_the_parasitic_weed_Phelipanche_aegyptiaca/24664671 |
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