Insect Cell Culture: Fundamental and Applied Aspects [electronic resource] /

Insect Cell Lines -- Development and characterization of insect cell lines -- New approaches to insect tissue culture -- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene -- Insect cell physiology -- Insect cell cultivation: growth and kinetics -- Medium design for insect cell culture -- Viruses -- Baculovirus-insect cell interactions -- Replication patterns and cytopathology of cells infected with baculoviruses -- Construction of baculovirus recombinants -- Passage effect of virus infection in insect cells -- Post-translational modifications in insect cells -- Productivity of insect cells for recombinant proteins -- Chaperone and foldase coexpression in the baculovirus-insect cell expression system -- Engineering Aspects -- Shear sensitivity of insect cells -- Insect cell bioreactors -- Perfusion bioreactors for the production of recombinant proteins in insect cells -- Immobilization of insect cells -- Modelling baculovirus infection of insect cells in culture -- Scale up aspects of sparged insect-cell bioreactors -- Oxygen gradients in small and big sparged insect-cell bioreactors -- Downstream processing of insect cell cultures -- Applications -- Parvovirus diagnostics and vaccine production in insect cells -- Classical swine fever virus diagnostics and vaccine production in insect cells -- Production of multidomain complement glycoproteins in insect cells -- Economic and Regulatory Aspects -- Economics of baculovirus-insect cell production systems -- Safety aspects of insect cell culture -- Regulatory issues in the use of insect-cell culture.

Bibliographic Details

Main Authors:	Vlak, J.M. editor., Gooijer, C.D. de. editor., Tramper, J. editor., Miltenburger, H.G. editor., SpringerLink (Online service)
Format:	Texto biblioteca
Language:	eng
Published:	Dordrecht : Springer Netherlands, 2002
Subjects:	Life sciences., Biotechnology., Biochemistry., Animal anatomy., Entomology., Life Sciences., Animal Anatomy / Morphology / Histology., Animal Biochemistry.,
Online Access:	http://dx.doi.org/10.1007/0-306-46850-6
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