Chemical composition of an anthelmintic fraction of Pleurotus eryngii against eggs and infective larvae (L3) of Haemonchus contortus
This study was aimed at evaluating thein vitroeffect of the edible mushroom (EM) Pleurotus eryngiiagainst the eggs and larvae (L3) of Haemonchus contortus. The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the ECS-1255 strain showed thehighest effect on mortality rates of L3 (18.83%) at 20 μg/mL. After subjecting this HA extract to a normal phase chromatography column, five fractions were obtained; fraction F5 (100% MeOH) was the most effective against eggs, with hatching inhibition percentages of 88.77 and 91.87% at 20 and 40 mg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) subjected this fraction to an acetylation reaction to determine the content of the secondary metabolites. The GC-MSanalysis showed that the F5 fraction was composed of trehalose CAS: 6138-23-4, polyols (L-iditol CAS: 488-45-9, galactitol CAS:608-66-2, D-mannitol CAS: 69-65-8, D-glucitol CAS: 50-70-4, and myoinositol CAS: 87-89-8), adipic acid CAS: 124-04-9, stearic acid CAS: 57-11-4, squalene CAS: 111-02-4, and β-sitosterol CAS: 83-46-5.
| Main Authors: | , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Pleurotus eryngii, Hongos comestibles, Composición química, Haemonchus contortus, Helmintos, Ovejas, Control de nemátodos, |
| Online Access: | https://www.hindawi.com/journals/bmri/2020/4138950/ |
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