A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to that of chemical synthesis for producing Ba1, a cysteine-rich spider neurotoxin. Methods: The transcription of the insecticidal neurotoxin Ba1 was obtained from a cDNA library of venom glands of the spider Brachypelma albiceps. It was cloned into the pCR®2.1-TOPO® cloning vector and then introduced in two different expression vectors, pQE40 and pET28a+. Each vector was transfected into E. coli M15 and BL21 cells, respectively, and expressed under induction with isopropyl thiogalactoside (IPTG). The chemical synthesis of Ba1 was performed in an Applied Biosystems 433A peptide synthesizer.
| Main Authors: | , , , , , |
|---|---|
| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Péptidos, Insecticidas, Brachypelma albiceps, Arañas, Síntesis química, |
| Online Access: | http://sii.ecosur.mx/Content/ProductosActividades/archivos/32243/textocompleto.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|