Selection of a nucleopolyhedrovirus for control of spodoptera frugiperda (Lepidoptera: noctuidae) structural, genetic, and biological comparison of four isolates from the Americas
Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is the principal pest of maize in tropical and subtropical regions of the Americas. Larvae of this species are susceptible to a nucleopolyhedrovirus (NPV) which has attracted interest as a potential biocontrol agent. Four strains of NPV isolated from infected S. frugiperda larvae in the United States, Nicaragua, and Argentina were subjected to a structural, genetic, and biological comparison to select a candidate isolate for use in biocontrol experiments in Mexico and Honduras. All isolates had an occlusion body polyhedrin protein of 32 kDa, but the virions of each isolate differed subtly in the pattern and abundance of certain structural polypeptides revealed by SDS-PAGE analysis. Restriction endonuclease analysis of viral DNA confirmed that these isolates were strains of a single virus species but showed that they were not genetically homogeneous; each isolate could be differentiated from the others using common restriction enzymes.
| Main Authors: | , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Spodoptera frugiperda, Salinas, Insecticidas biológicos, Baculoviridae, Control biológico de plagas, |
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