Potential use of SSR markers for Coffea spp. genetic mapping
Microsatellites, also called simple sequence repeats (SSRs), are versatile molecular markers that are widely spread in plant and animal genomes. Their interest resides in the fact that they are codominant and locus-specific. Among plants, they have been used to establish and saturate genetic maps for less than 10 years, in complement to RFLP, AFLP, RAPD, and isoenzymes. SSRs have already been used for coffee genetic studies; here, they will also allow good definition of quantitative trait loci that will be looked for in different progenies. A Coffea canephora (clone 126) genomic DNA library has been obtained using Rsa 1 endonuclease and enriched with GA and GT sequences using streptavidin-coated magnetic particles. More than 800 clones have been obtained and sequenced. After elimination of redundants, several hundreds of primer pairs are expected to be designed and be effectively available for detecting SSR loci by PCR amplification. Those loci will be mapped for Coffea inter and intraspecific crosses.
Main Authors: | , , , , , , , , |
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Format: | biblioteca |
Published: |
Trieste (Italia) ASIC
2001
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Subjects: | COFFEA CANEPHORA, MAPAS GENETICOS, MARCADORES GENETICOS, ISOENZIMAS, LOCI, MICROSATELITES, GENETICA MOLECULAR, SECUENCIA NUCLEOTIDICA, ADN POLIMORFICO AMPLIFICADO AL AZAR, POLIMORFISMO, |
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