Potential use of SSR markers for Coffea spp. genetic mapping

Microsatellites, also called simple sequence repeats (SSRs), are versatile molecular markers that are widely spread in plant and animal genomes. Their interest resides in the fact that they are codominant and locus-specific. Among plants, they have been used to establish and saturate genetic maps for less than 10 years, in complement to RFLP, AFLP, RAPD, and isoenzymes. SSRs have already been used for coffee genetic studies; here, they will also allow good definition of quantitative trait loci that will be looked for in different progenies. A Coffea canephora (clone 126) genomic DNA library has been obtained using Rsa 1 endonuclease and enriched with GA and GT sequences using streptavidin-coated magnetic particles. More than 800 clones have been obtained and sequenced. After elimination of redundants, several hundreds of primer pairs are expected to be designed and be effectively available for detecting SSR loci by PCR amplification. Those loci will be mapped for Coffea inter and intraspecific crosses.

Bibliographic Details

Main Authors:	63557 Dufour, M., 75865 Hamon, P., 99614 Noirot, M., 110931 Risterucci, A.M., 51366 Brottier, P., 128642 Vico, V., 86193 Leroy, T., 3180 Association Scientifique Internationale du Cafe, París (Francia), 32308 19. International Scientific Colloquium on Coffee Trieste (Italia) 14-18 May 2001
Format:	biblioteca
Published:	Trieste (Italia) ASIC 2001
Subjects:	COFFEA CANEPHORA, MAPAS GENETICOS, MARCADORES GENETICOS, ISOENZIMAS, LOCI, MICROSATELITES, GENETICA MOLECULAR, SECUENCIA NUCLEOTIDICA, ADN POLIMORFICO AMPLIFICADO AL AZAR, POLIMORFISMO,
Tags:	Add Tag No Tags, Be the first to tag this record!