Characterization of Rhizoctonia solani, the causal agent of damping-off on seedlings of coffee
The anastomosis group of R. solani, associated with hypocotyl rot and damping-off of coffee seedlings, was determined based on the characterization of the number of nuclei per cell, hyphal anastomosis reaction, RAPD markers, ITS-5.8s rDNA nucleotidesequence and pathogenicity of two R. solani isolates (RsC1 and RsC2). Both isolates showed 4-9 nuclei per cell. On the basis of the hyphal anastomosis reaction, RsC1 was found to belong to AG-4 HGI and RsC2 to AG-4 HGI and AG-4 HGII. A genetic similarityof 0.7 was observed between RsC1 and RsC2 isolates, and genetic similarities of 0.7 and 0.6 were observed between R. solani isolates of coffee (RsC1 and RsC2) and the AG-4 HGI tester, on the basis of RAPD analysis. RsC1 was 99.4 percent similar to the Ag-4 HGI tester based on the ITS-5.8s rDNA nucleotide sequence analysis. Both isolates were confirmed to be R. solani AG-4 HGI and both were pathogenic to seedlings, causing lesions on the hypocotyl.
| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
2001
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| Subjects: | COFFEA, RHIZOCTONIA SOLANI, ENFERMEDADES FUNGOSAS, MARCADORES GENETICOS, HIPOCOTILOS, ADN, SECUENCIA NUCLEOTIDICA, PATOGENICIDAD, ENFERMEDADES DE LAS PLANTAS, ORGANISMOS PATOGENOS, ADN POLIMORFICO AMPLIFICADO AL AZAR, ARN RIBOSOMIAL, PLANTULAS, |
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