Detection of genetic diversity and selective gene introgression in coffee using RAPD markers
RAPD (ramdomly amplified polymorphic DNA) markers generated by arbitary decamers have been succssfully employed to detect genetic plymorphisms between coffee species and between Coffea arabica genotypes. The RAPD profiles were used to construct dendrograms and these were consistent with the known history and evolution of Coffea arabica. Material originating from Ethiopia and the arabica sub-groups - C. arabica var. rypica and C. arabica var bourbon - were clearly distinguished. RAPD analysis therefore reflects morphological differences between the sub-groups and the geographical origin of the coffee material. Species-specific amplification products were also identified, but, more importantly, amplification products specific to C. canephora were identified in two C. arabica genotypes, Rume Sudan and Catimor 5175. This diagnostic produc is therefore indicative of interspecific gene flow in coffee and has biological implications for selective introgressive hybridisation in coffee. Our study demonstrates the power of the polymerase chain reaction technology for the generation of genetic markers for long-lived perennial tree and bush crops.
| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
1994
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| Subjects: | COFFEA ARABICA, MARCADORES GENETICOS, GENOTIPOS, INTROGRESION, HIBRIDACION, |
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