Effect of plant growth regulators on somatic embryogenesis in leaf cultures of Coffea canephora
The effects of plant growth regulators on somatic embryogenesis were studied in leaf cultures of Coffea canephora. The maximum number of somatic embryos were obtained on media that contained only cytokinin as a plant growth regulator. All of the auxins tested (NAA, IBA, IAA and 2,4-D) inhibited the formation of embryos. The optimal concentration of each cytokinin (2-iP, BA and kinetin) for somatic embyogenesis was 5 ¿M. Under optimal conditions, each explant formed more than 100 embryoids with little callus and few adventitious roots. Embryoids were formed only at the cut edges of the leaf discs. Cytokinins were absorbed only at the cut edges of leaf discs that were in contact with the medium, and were not transported to other parts of the explant
| Main Authors: | , , , , |
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| Format: | biblioteca |
| Published: |
1991
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| Subjects: | COFFEA CANEPHORA, EMBRIOGENESIS SOMATICA, CULTIVO DE TEJIDOS, ESTIMULANTES DEL CRECIMIENTO, |
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