Minimal growth in vitro conservation of coffee (Coffea spp.), 1: Influence of low concentrations of 6-benzyladenine
We have studied the influence of low concentrations of 6-benzyladenine on growth limitation, in order to preserve coffee germplasm through a microcutting collection. Concentrations of 0 ¿M, 1.3 ¿M and 4.4 ¿M were compared in four species: Coffea congensis, C. canephora, C. liberica and C. racemosa. After six months, microcutting behaviour varied between the different treatments, and a species effect was observed. The slow growing species (C. liberica and C. congensis) needed 1.3 ¿M; the others coffee species (C. canephora and C. racemosa) exhibited moderate caulogenesis on 6-benzyladenine-free medium. Zero and low concentrations did not affect survival rates. In conclusion 1.3 ¿M seems most appropriate for conserving all four species.
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| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
1991
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| Subjects: | COFFEA, CULTIVO IN VITRO, SUSTANCIAS DE CRECIMIENTO VEGETAL, 6-BENZILADENINA, CONSERVACION DEL GERMOPLASMA, MICROESTACAS, DOSIS DE APLICACION, MERISTEMAS, |
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