Utilization de phytorégulateurs de croissance pour l'induction in vivo de bourgeons axillaires latents de Coffea arabica v. Catimor et leur propagation in vitro
The main aim of this work was to know whether technology could be improved by using material directly from the field, which should make it possible to: use the largest possible amount of plant material (up to the fifth or sixth node); save time by inducing early break of the axillary buds. For this purpose, different doses of BAP (0 to 1000 ppm) and BIA (0 - 100 ppm) were tried. They were applied on the different nodes ( 1 to 5) on whole cuttings or cuttings that had been cut in half. The results obtained tend to show that a large proportion of the plant material (dormant bud) can be recuperated on nodes 3, 4 and 5, in a relatively short time (one month). The buds break between the roth and 12th day, and the orthotropic axes are recuperated in vitro between the 30th and 35th day (according to their size). After 40 days, the number of buds that break falls according to the node level involved. The buds break later on the 1st and 2nd nodes (due to the high apical dominance of the terminal bud). With the in vitro methods used, 65 to 70 per cent of the explants were recuperated. The results obtained show that micro-propagation of coffee has a practical application which involves using material directly from the field, without a period under glass, and greater use can be made of the potentialities of the plant (N3, N4, N5, N6, etc) in the field
| Main Authors: | , , , , , |
|---|---|
| Format: | biblioteca |
| Published: |
París (Francia)
1988
|
| Subjects: | COFFEA ARABICA, CRUZAMIENTO, ESTIMULANTES DEL CRECIMIENTO, YEMAS AXILIARES, MICROPROPAGACION, CATIMOR, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|