Characterisation of cacao germplasm using isoenzyme markers, 1: A preliminary survey of diversity using starch gel electrophoresis and standardisation of the procedure
A preliminary screening of isoenzymes in cacao has been carried out with a view to the use of the data in characterising cacao germplasm. Staining was carried out for 24 enzyme systems in leaf tissue. Seventeen of these showed activity, and in seven enzyme systems, reproducible variation in banding patterns was obtained. A single enzyme, peroxidase, was extracted from woody tissue and also exhibited repeatable variation. It was considered that by running extracts from leaves and wood on starch gels, and staining for these 8 enzyme systems, valuable information would be obtained. This could be used to identify clonal material for breeding. It could also be used to supplement the usual morphological descriptors for characterising material in genebanks.
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
Nov
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| Subjects: | THEOBROMA CACAO, CONSERVACION DEL GERMOPLASMA, RECURSOS GENETICOS, ISOENZIMAS, SISTEMAS ENZIMATICOS, NORMALIZACION, |
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