Initiation and growth of callus and cell suspensions of Theobroma cacao L.
Callus and suspension cultures of Theobroma cacao L., initiated form immature cotyledons of beans from pods harvested 120-130 days after pollination were established. A modified B-5 or Murashige-Skoog agar medium sustained growth of callus without loss of vigour after each sub-culture. A 15 fold weight increase occurred during the 4 weeks culture periods at 30 more less 1 centigrade. Coconut water improved callus growth substantially. The optimum hormonal concentrations for growth of suspension were 0.5 mg 1 exponent -1 of 2,4-dichlorophenoxyacetic acid and 0.1 mg 1 exponent -1 of kinetin in a Murashige-Skoog basal medium liquid medium. The optimum temperature for growth of suspensions was 25-30 centigrade grade. The cell number and cell mass of suspensions increased 20-fold in 14 days. No organogenesis or embryogenesis was observed
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| Format: | biblioteca |
| Published: |
1981
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| Subjects: | CULTIVO DE TEJIDOS, THEOBROMA CACAO, CULTIVO DE CELULAS, CALLO, CULTIVO EN SUSPENSION, COTILEDONES, MEDIO DE CULTIVO, |
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