Screening survey samples for the presence of barley yellow dwarf viruses
In Purdue University laboratory, enzyme-linked immunosorbent assay (ELISA) is the most successful procedure for routine surveys of the ocurrence of barley yellow dwarf virus (BYDV). Polyclonal antisera are used but the potential of monoclonal antibodies with defined reactivity is being considered. Handling and extraction procedures for samples are described. Some host variables that have to be considered in sample analysis are: different hosts of BYDV differ considerably in virus content; some resistant cereal cultivars contain much smaller amounts of virus than susceptible ones; virus content varies with age at infection and with incubation time; roots usually contain more virus than leaves; virus yields depend on sample condition and storage; virus content can vary among different leaves of the same plant. In surveys, ELISA activity survives well in leaf tissue even if it is frozen or dried, a condition that makes it feasible to transport samples for testing. However, information on the reduction of virus reactivity or cross-reactivity to BYDV antisera is needed for samples after transportation. Transfer of dry leaf tissue presents no quarantine hazard. (EN)
| Main Authors: | , , , , , , |
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| Format: | biblioteca |
| Published: |
México, D.F. (México)
1984
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| Subjects: | DIAGNOSTICO, HORDEUM VULGARE, VIRUS ENANISMO AMARILLO DE CEBADA, VIROSIS, ELISA, AISLAMIENTO (TECNICA), TECNICAS INMUNOENZIMATICAS, ESTADOS UNIDOS DE AMERICA, |
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