Diagnosis of Mycobacterium bovis and Mycobacterium paratuberculosis infections in cattle by in vitro lymphocyte immunostimulation
Peripheral blood lymphocytes from one calf artificially infected with Mycobacterium bovis and one cow with naturally occurring infection of Mycobacterium paratuberculosis were incubated in vitro with purified protein derivatives (PPD) of M. bovis, M. paratuberculosis, M. avium, and M. tuberculosis and the degree of lymphocyte stimulation by the PPD was determined according to the incorporation of / 3H/ -thymidine (3HdT) in deoxyribonucleic acid (DNA). Peripheral blood lymphocytes from infected cattle were stimulated as much as 15-fold by the homologous PPD prepared from the mycobacterial species causing infection, whereas heterologous PPD induced little or no stimulation. Lymphocytes from control cattle were unaffected
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| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
May
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| Subjects: | DIAGNOSTICO DE LABORATORIO, MYCOBACTERIUM, MYCOBACTERIUM PARATUBERCULOSIS, DERIVADO PROTEICO PURIFICADO, ACIDOS NUCLEICOS, BACTERIOSIS, LINFOCITOS, RESPUESTA INMUNOLOGICA, GANADO BOVINO, |
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