Application of a modified enzyme-linked immunorbent assay for detecting mycobacterial antibodies in the sera of cattle from a herd in which Mycobacterium bovis infection was diagnosed :
A routine enzyme-linked immunosorbent assay (ELISA) and a modified ELISA were used for detecting mycobacterial antibodies in the sera of cattle in a herd in which Mycobacterium bovis infection was diagnosed previously. Affinity purified goat-antibovine IgG (H+L) labeled with horseradish peroxidase was used as the conjugate and M. bovis purified protein derivative (PPD) tuberculin was used as the antigen. Improved results were obtained in detecting mycobacterial antibodies in the sera of tuberculous cattle when a modified ELISA with carbodiimide and NH4Cl was used. Reactions on modified ELISA were detected in sera of 77 of 80 adult cows responding on tuberculin skin tests conducted in the vulva and/or caudal fold and on comparative cervical tuberculin skin tests; 43 cattle had gross lesions at slaughter. Moreover, ELISA reactions were detected in sera of 44 of 46 cattle responding on tuberculin skin tests conducted in the vulva and/on caudal fold and negative on comparative cervical tuberculin skin tests; 20 cattle had gross lesions. ELISA reactions were also detected in sera of 24 of 196 cattle negative on tuberculine skin tests conducted on the vulva and/or caudal fold and not receiving comparative cervical tuberculin tests
| Main Authors: | , , , , , , |
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| Format: | biblioteca |
| Language: | eng |
| Published: |
Richmond, Va. (EUA)
1983
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| Subjects: | TECNICAS INMUNOENZIMATICAS, ELISA, DIAGNOSTICO, MYCOBACTERIUM, DERIVADO PROTEICO PURIFICADO, TUBERCULINA, ANTICUERPOS, BACTERIOSIS, GANADO BOVINO, TUBERCULOSIS BOVINA, PRUEBA TUBERCULINICA, LESIONES, |
| Online Access: | http://orton.catie.ac.cr/repdoc/ |
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