Description and evaluation of a enzyme-linked immunosorbent assay test used for brucellosis screening of dairy cattle in Southern California
An enzyme-linked immunosorbent assay (ELISA) for brucellosis screening was modified by using standard tube test antigen. This test was compared to the rapid screening test (RST) in two cattle populations: (a) infected herds and (b) market cattle samples. The samples were further tested by the card, rivanol, and complement-fixation tests. In the infected herd data, 19.835 records were evaluated from nine herds containing 7.889 cattle (X=876.7). A total of 122 reactors were found (0.615 per cent of records on 1.55 per cent of cattle). Of the RST negative samples, 641 were ELISA positive of which eight were reactors. Conversely, of the ELISA negative samples, there were 601 RST positive, of which two were reactors. The difference was statiscally significant. There were 3,847 routine MCI samples tested by both RST and ELISA. Supplemental tests revealed 14 reactors. One reactor was detected by ELISA alone; the other 13 were found by both tests. A one-character computer reporting scheme is reported and several screening levels are discussed. Under the conditions of this study, the ELISA appeared to be superior to the RST
| Main Authors: | , , , , |
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| Format: | biblioteca |
| Published: |
Richmond, Va. (EUA)
1986
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| Subjects: | TECNICAS INMUNOENZIMATICAS, BRUCELOSIS, BACTERIOSIS, DIAGNOSTICO DE LABORATORIO, ELISA, GANADO DE LECHE, CALIFORNIA, |
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