Quality control of four Rev. 1 antibrucella vaccines

Four lyopholized strains of Brucella melitensis Rev. 1 vaccine were compared for in vitro resistance to penicillin and streptomycin, in vivo persistence after vaccination and immunogenicity in a mouse model. The four strains had the growth characteristics described for the original one. They grew slowly on Trypticase Soy Agar medium, were resistant to 2.5 ug of streptomycin and inhibited by 5 units of penicillin. However, detailed analysis according to concentration of both antibiotics evidenced differences between strains. Persistence in the spleen of mice after vaccination depended on the strain inoculated. All strains were highly immunogenic. However, one of them, strain 4, induced comparatively lower immunity. There was no clear correlation between persistence after vaccination and immunogenicity

Bibliographic Details

Main Authors:	105946 Plommet, M. eds., 50339 Bosseray, N. Institut National de la Recherche Agronomique, Nouzilly (Francia), 128472 Verger, J.M., 5846 Commision of the European Communities, Luxemburgo (Bélgica)
Format:	biblioteca
Published:	Dordrecht (Países Bajos) Martinus Nijhoff 1985
Subjects:	BRUCELOSIS, BRUCELLA MELITENSIS, VACUNA, CALIDAD, PENICILINA, ESTREPTOMICINA,
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